Triterpenes from Centella asiatica stimulate extracellular matrix accumulation in rat experimental wounds

Citation
Fx. Maquart et al., Triterpenes from Centella asiatica stimulate extracellular matrix accumulation in rat experimental wounds, EUR J DERM, 9(4), 1999, pp. 289-296
Citations number
24
Categorie Soggetti
da verificare
Journal title
EUROPEAN JOURNAL OF DERMATOLOGY
ISSN journal
11671122 → ACNP
Volume
9
Issue
4
Year of publication
1999
Pages
289 - 296
Database
ISI
SICI code
1167-1122(199906)9:4<289:TFCASE>2.0.ZU;2-Y
Abstract
Titrated Extract from Centella asiatica (TECA) is a drug which has been use d for many years in Europe for the treatment of wound healing defects. It i s a reconstituted mixture of 3 triterpenes extracted from the plant, asiati c acid, madecassic acid and asiaticoside. In this report, we studied the ef fects of TECA and its separated components in the wound chamber model descr ibed by Schilling ct al. Stainless steel wound chambers were surgically ins erted under the skin of rats and received serial injections of either TECA or its purified components. Chambers were collected at days 7, 14, 21 or 28 for biochemical analysis or histological examination. TECA-injected wound chambers were characterized by increased dry weight, DNA, total protein, co llagen and uronic acid contents. Peptidic hydroproline was also increased, showing an increased remodeling of the collagen matrix in the wound. The 3 purified components of TECA were all able to reproduce the effects of the c omplete drug, with some differences depending on the product. Asiatic acid and asiaticoside were the most active of the 3 triterpenes. Asiaticoside ex erted a preferential stimulation of collagen synthesis and was active at lo w doses only. In addition to collagen, the 3 components were also able to s timulate glycosaminoglycan synthesis.