Immobilized metal-ion affinity chromatography of peptides on metalloporphyrin stationary phases

The retention of Selected dipeptides and tripeptides containing tyrosine wa s examined; As stationary phase an aminopropylated silica gel loaded with c ovalently linked tetraphenylporphyrin was used. The effect of metalization of porphyrin with Cu(II) and Zn(II) on retention was investigated. The obse rved separation is based on a mixed mechanism involving pi-pi and hydrophob ic interactions as well as complex formation between immobilized metal ions and peptides. A satisfactory separation was demonstrated for C-peptide and bovine insulin. The possibility of:separation of various insulins was also investigated.