Purpose: The purpose of this study was to evaluate the difference between f
ull chart, single line, and single optotypes visual acuity (VA) test result
s in healthy and amblyopic children. Methods: Thirty-five children with amb
lyopia (20 with strabismus and 15 with anisometropia) and 40 ophthalmologic
ally normal age-matched children were examined. The mean age of the patient
s in the study and control groups did not differ significantly (P=.9). A co
mmercial projector that projected tumbling-E randomly placed optotypes was
used. The VA of the amblyopic eye of the patients in the study group and th
e right eye of the patients in the control group was examined first using a
full chart of optotypes, then using a single line of optotypes, and finall
y with individual symbols. The procedure was repeated with the other eye. R
esults: LogMAR VA improved when the full chart was substituted with a singl
e line, and improved by a similar increment further with single optotypes,
in both the study and control groups. VA improved significantly more in eye
s with amblyopia than in control subjects. Results were not influenced by a
ge. Conclusion: VA testing using a single line gives better, sometimes misl
eading results, than tests with a full chart because it reduces but does no
t eliminate the crowding effect. When using a device that can employ more t
han 1 mode, the exact test mode should be specified and maintained througho
ut the follow-up.