Evaluation of the cytocompatibility of three endodontic materials

The goal of this in vitro study was to evaluate the relative cytocompatibil ity of three endodontic materials: calcium hydroxide, a calcium oxide-based compound, and a zinc oxide-eugenol-based sealer. The evaluation was conduc ted 24, 72, and 168 h after contact with the compounds and involved three c omplementary techniques: a colorimetric cytotoxicity test, scanning electro n microscopy, and flow cytometry, The results we obtained confirmed the ini tial cytotoxicity of the zinc oxide-eugenol-based sealer and showed that th e calcium oxide-based compound had the same relative cytocompatibility as c alcium hydroxide.