Normal and reversed phase thin layer chromatography of selected 2,5-anhydroaldohexose ethylene acetal derivatives

The chromatographic behavior of 31 samples of variously substituted 2,5-anh ydroaldohexose ethylene acetal derivatives has been studied on silica gel a nd C-18 modified silica gel layers with, respectively, binary non-aqueous a nd aqueous mobile phases. The slopes and intercepts of the linear relations hips between the retention constant (R-M) and the logarithm of the volume f raction of the diluent in non-aqueous mobile phase, as well as of the volum e fraction of organic component in aqueous-organic mobile phase, have been calculated and are discussed in relation to solute and mobile and stationar y phase characteristics. The retention and relative retention of compounds depend largely on the retention behavior of their substituents.