A new high performance thin-layer chromatography (HPTLC) method for determination of theophylline in plasma

A new high-performance thin-layer chromatography (HPTLC) method for the det ermination of theophylline in plasma has been developed. Plasma samples wer e prepared by the internal reference method. Denaturation was carried out t hrough cold centrifugation. Separation of the drug from the complex matrix was accomplished within a sh ort 50 mm migration distance and 25 min migration time on silica gel 60 F 2 54 layers using toluene-isopropanol-acetic acid (80:20:5) as the mobile pha se. The analyte was quantified by absorbance/reflectance densitometry using peak-area ratio analysis. Validation parameters were investigated consider ing the special features of the method.