Mitochondrial DNA was used to infer the phylogeny and genetic divergences o
f Chrysomya albiceps (Wiedemann) and C. rufifacies (Maquart) specimens from
widely separated localities in the Old and New World. Analyses based on a
2.3-kb region including the genes for cytochrome oxidase subunits I and II
indicated that the 2 species were separate monophyletic lineages that have
been separated for >1 million years. Analysis of DNA, in the form of either
sequence or restriction fragment-length polymorphism (RFLP) data, will per
mit the identification of problematic specimens.