Absorption of IgG does not enhance toxoplasma IgM and IgA immunoblotting

Total IgG, IgM and IgA levels and toxoplasma IgG, IgM and Igli immunoblotti ng patterns were assayed in 10 sera before and after IgG absorption with Pr otein G - Sepharose 4, Removal of IgG (mean reduction 96%) was accompanied by a significant reduction in the level of IgM (mean reduction 56%) and IgA (mean reduction 53%) in nine of the 10 sera. The absorbed supernates showe d fewer and weaker IgM bands in five sera, but IgA immunoblotting patterns were unaffected by absorption. There was no benefit in removing IgG in toxo plasma IgM and IgA immunoblotting.