R. Butowt et al., Involvement of JIM13-and JIM8-responsive carbohydrate epitopes in early stages of cell wall formation, J PLANT RES, 112(1105), 1999, pp. 107-116
We studied the possible involvement of JIM13- and JIM8-responsive epitopes
in the formation of the cell wall at the surface of regenerating protoplast
s of sugar beet (Beta vulgaris L). The spatial and temporal expression of b
oth antigens was studied in suspension cells used as the source-tissue for
protoplast isolation, in suspension- and mesophyll-derived protoplasts, and
in cells which developed from both types of protoplast. Immunofluorescence
and immunocytochemical-electron microscopic methods revealed that labeling
was present in the cell walls of most suspension cells and also in the inc
ipients of cell walls synthesized around the protoplasts. This signal becam
e much more intense as rebuilding of the cell wall progressed during cultur
e. Relatively weaker labeling was observed in the cytoplasm, where it was f
requently associated with the vacuolar compartment. Signal intensity varied
between individual cells of the same population and in successive stages o
f development, but was always stronger with JIM13 than with JIM8. The role
of JIM13-responsive epitope in the development of suspension-derived protop
lasts was further studied by its ability to bind antibody added to cultures
of different ages. Both JIM8- and JIM13-responsive epitopes were widesprea
d in sugar beet cells of different origin and stage of cell wall synthesis.
These epitopes may play an important role in cell wall formation and growt
h under in vitro conditions.