Effect of cefodizime, ofloxacin, ciprofloxacin and interferon alpha-2a, alone and in combination, on phagocytic and candidacidal functions of leucocytes from patients with chronic renal failure

We have examined the effects of cefodizime, ofloxacin, ciprofloxacin and in terferon alpha-2a (IFN alpha-2a), alone and in combination, on phagocytosis and killing of Candida albicans blastospores by polymorphonuclear leucocyt es (PMNs) from patients with chronic renal failure. The effects of therapeu tic concentration of each drug on the phagocytic and candidacidal capacity were studied in vitro. 20 chronic renal failure patients, average age 45, w ere included. Phagocytosis and candidacidal capacity were significantly enh anced (P < 0.001) after pre-treatment of PMNs with cefodizime at 10 mu g/ml , but ofloxacin (4 mu g/ml) and IFN alpha-2a (10 U/ml) had no effect compar ed to the control with no antibiotics. However, only candidacidal capacity increased significantly with ciprofloxacin at 2 mu g/ml (P < 0.001). In con trast, ciprofloxacin had no significant effect on phagocytosis (P > 0.05). Combinations of these three drugs with IFN alpha-2a produced the same effec t as these two drugs alone. These results indicate that cefodizime, ofloxac in and ciprofloxacin alone and in combination with IFN alpha-2a at safely a chievable concentrations generally do not suppress PMN function in vitro. M ed Sci Res 27:315-318 (C) 1999 Lippincott Williams Wilkins.