Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam

Citation
T. Miyazawa et al., Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam, MICROB IMMU, 43(6), 1999, pp. 609-612
Citations number
20
Categorie Soggetti
Microbiology
Journal title
MICROBIOLOGY AND IMMUNOLOGY
ISSN journal
03855600 → ACNP
Volume
43
Issue
6
Year of publication
1999
Pages
609 - 612
Database
ISI
SICI code
0385-5600(1999)43:6<609:IOFPFP>2.0.ZU;2-C
Abstract
Feline parvovirus (FPV) was isolated rather frequently from the peripheral blood mononuclear cells (PBMCs) of cats in northern Vietnam by coculturing with MYA-1 cells (an interleukin-2-dependent feline T lymphoblastoid cell l ine) or Crandell feline kidney (CRFK) cells (a feline renal cell line). Eff iciency of virus isolation was higher in MYA-1 cells than in CRFK cells. In terestingly, among the 17 cats from which FPV was isolated, 9 cats were pos itive for virus neutralizing (VN) antibody against FPV, indicating that FPV infected PBMCs and was not eliminated from PBMCs even in the presence of V N antibodies in the cats.