Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam

Feline parvovirus (FPV) was isolated rather frequently from the peripheral blood mononuclear cells (PBMCs) of cats in northern Vietnam by coculturing with MYA-1 cells (an interleukin-2-dependent feline T lymphoblastoid cell l ine) or Crandell feline kidney (CRFK) cells (a feline renal cell line). Eff iciency of virus isolation was higher in MYA-1 cells than in CRFK cells. In terestingly, among the 17 cats from which FPV was isolated, 9 cats were pos itive for virus neutralizing (VN) antibody against FPV, indicating that FPV infected PBMCs and was not eliminated from PBMCs even in the presence of V N antibodies in the cats.