The lignin biodegradation process has an important role in the carbon
cycle of the biosphere. The study of this natural process has develope
d mainly with the use of basidiomycetes in laboratory investigations.
This has been a logical approach since most of the microorganisms invo
lved in lignocellulosic degradation belong to this class of fungi. How
ever, other microorganisms such as ascomycetes and also some bacteria,
are involved in the lignin decaying process. This work focuses on lig
nin biodegradation by a microorganism belonging to the ascomycete clas
s, Chrysonilia sitophila. Lignin peroxidase production and characteriz
ation, mechanisms of lignin degradation (lignin model compounds and li
gnin in wood matrix) and biosynthesis of veratryl alcohol are outstand
ing. Applications of C. sitophila for effluent treatment, wood biodegr
adation and single-cell protein production are also discussed.