Male accessory sex gland secretions affect oocyte Ca2+ oscillations duringin-vitro fertilization in golden hamsters

To evaluate the effect of male accessory sex gland secretions on Ca2+ oscil lations of oocytes, epididymal or ejaculated spermatozoa recovered from ute ri were used to inseminate oocytes. Ca2+ oscillations were measured by Fura 2 fluorescence imaging (F340/F380). We showed that although Ca2+ oscillati ons induced by ejaculated spermatozoa had a pattern similar to those induce d by epididymal spermatozoa, the amplitude of the first Ca2+ transient in t he former group was significantly higher (P < 0.05) and the duration was si gnificantly longer (P < 0.01). Oocytes inseminated with ejaculated spermato zoa recovered from uteri from males had ampullary glands or ventral prostat es removed showed significantly lower Ca2+ oscillations compared to the con trols (P < 0.05, P < 0.01 respectively). Moreover, the relative area of the first Ca2+ transient in treatment groups was significantly smaller than th e control. In addition, a significantly higher percentage of oocytes (52%) inseminated by spermatozoa from males with all accessory sex glands removed showed non-oscillatory Ca2+ transients, compared to the controls (5%, P< 0 .05). These results indicate that accessory sex gland secretions can affect Ca2+ oscillations. The differences between Ca2+ oscillations induced by ep ididymal and uterine spermatozoa from males with all accessory sex glands r emoved suggest that uterine factors may also influence this process.