THE CULTURE OF CHICKEN-EMBRYO FIBROBLAST CELLS ON MICROCARRIERS TO PRODUCE INFECTIOUS BURSAL DISEASE VIRUS

The cultures of chicken embryo fibroblast (CEF) cells in flasks, spinn er bottles, and bioreactors were studied. The growth and metabolism ch aracteristics of CEF cells and the feasibility of the CEF cell culture in bioreactor were investigated. The plating process of the CEF cells on GT-2 microcarriers in spinner bottles was studied, and a plating k inetic model was presented. The culture of CEF cells in 1.5 L CelliGen bioreactor to produce infectious bursal disease virus (IBDV) had met success. Whereas the additive microcarriers were fed during the cultur e, the cell density was increased 10 times as against seed cells adher ing to microcarriers and the virus titer was as high as 7.5. All the a forementioned experimental results have laid the foundation for high d ensity culture of CEF cells and process scale-up.