LACK OF EFFECT OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR ONSECRETION OF INTERFERON-TAU, OTHER PROTEINS, AND PROSTAGLANDIN E-2 BYTHE BOVINE AND OVINE CONCEPTUS

Three experiments tested the effects of recombinant bovine granulocyte -macrophage colony-stimulating factor (rbGM-CSF) on the preimplantatio n bovine and ovine conceptus. There was no effect of rbGM-CSF on the s ecretion of total radiolabeled protein in conditioned medium, immunore active interferon-tau (IFN tau), antiviral activity, or prostaglandin E-2 from Day 16-18 bovine conceptuses cultured for 24 hr with [H-3]leu cine and +/-10 ng/ml rbGM-CSF. Similarly, there was no effect of 1 ng/ ml rbGM-CSF on the secretion of total radiolabeled protein, IFN tau, o r antiviral activity from Day 17 ovine conceptuses. There was also no beneficial effect of 1 or 10 ng/ml rbGM-CSF on the presence of immunor eactive IFN tau in conditioned medium from in vitro-produced bovine bl astocysts at Day 7-8 after fertilization. Results indicate that IFN ta u secretion from bovine and ovine conceptuses are unresponsive to rbGM -CSF at the concentrations tested. (C) Elsevier Science Inc. 1997.