Isochorismate synthase isoforms from elicited cell cultures of Rubia tinctorum

Authors
van Tegelen, LJP Bongaerts, RJM Croes, AF Verpoorte, R Wullems, GJ
Citation
Ljp. Van Tegelen et al., Isochorismate synthase isoforms from elicited cell cultures of Rubia tinctorum, PHYTOCHEM, 51(2), 1999, pp. 263-269
Citations number
22
Categorie Soggetti
Agricultural Chemistry","Animal & Plant Sciences
Journal title
PHYTOCHEMISTRY
ISSN journal
00319422 → ACNP
Volume
51
Issue
2
Year of publication
1999
Pages
263 - 269
Database
ISI
SICI code
0031-9422(199905)51:2<263:ISIFEC>2.0.ZU;2-I
Abstract
Elicitation of Rubia tinctorum cell cultures with a Pythium aphanidermatum elicitor leads to a doubling of anthraquinone content which is preceded by a large rise in isochorismate synthase (ICS; EC 5.4.99.6) activity. Two ICS isoforms were purified from the elicited cultures. Both isoforms had an ab solute requirement for Mg2+. Isoform I and II had a K-m for chorismate of 3 65 and 466 mu M, respectively. Gel filtration indicated a molecular mass of approximately 67 kD for both isoforms. (C) 1994 Elsevier Science Ltd. All rights reserved.