Circular dichroism analysis of insect cell expressed herpes simplex virus type I single-stranded DNA-binding protein ICP8

Authors
Spatz, M Ali, SA Auer, M Graf, C Eibl, MM Steinkasserer, A
Citation
M. Spatz et al., Circular dichroism analysis of insect cell expressed herpes simplex virus type I single-stranded DNA-binding protein ICP8, PROT EX PUR, 16(1), 1999, pp. 40-46
Citations number
39
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PROTEIN EXPRESSION AND PURIFICATION
ISSN journal
10465928 → ACNP
Volume
16
Issue
1
Year of publication
1999
Pages
40 - 46
Database
ISI
SICI code
1046-5928(199906)16:1<40:CDAOIC>2.0.ZU;2-5
Abstract
We have developed a baculovirus expression system for the rapid and efficie nt production of large quantities (>5 mg/10(8) cells) of ICP8. The recombin ant ICPS is fully functional and binds to single-stranded DNA. Secondary st ructure calculations from circular dichroism measurements indicate a conten t of 34.5% or-helix and 15.4% beta-sheet. This is the first structural repo rt for ICP8 using CD analysis, which will be very useful for high-throughpu t assay development and mechanistic studies, (C) 1999 Academic Press.