Association between G(2)-phase block and repair of radiation-induced chromosome fragments in human lymphocytes

Citation
M. Durante et al., Association between G(2)-phase block and repair of radiation-induced chromosome fragments in human lymphocytes, RADIAT RES, 151(6), 1999, pp. 670-676
Citations number
27
Categorie Soggetti
Experimental Biology
Journal title
RADIATION RESEARCH
ISSN journal
00337587 → ACNP
Volume
151
Issue
6
Year of publication
1999
Pages
670 - 676
Database
ISI
SICI code
0033-7587(199906)151:6<670:ABGBAR>2.0.ZU;2-D
Abstract
We have studied the induction of chromosomal aberrations in human lymphocyt es exposed in G(0) to X rays or carbon ions. Aberrations were analyzed in G (0), G(1), G(2) or M phase. Analysis during the interphase was performed by chemically induced premature chromosome condensation, which allows scoring of aberrations in G(1), G(2) and M phase; fusion-induced premature chromos ome condensation was used to analyze the damage in G(0) cells after incubat ion for repair; M-phase cells were obtained by conventional Colcemid block. Aberrations were scored by Giemsa staining dr fluorescence in situ hybridi zation (chromosomes 2 and 4). Similar yields: of fragments were observed in G(1) and G(2) phase, but lower yields were scored in metaphase,The frequen cy of chromosomal exchanges was similar in G(0) (after repair), G(2) and M phase for cells exposed to X rays, while a lower frequency of exchanges was observed in M phase when lymphocytes were irradiated with high-LET carbon ions,The results suggest that radiation-induced G(2)-phase block is associa ted with unrejoined chromosome fragments induced by radiation exposure duri ng G(0). (C) 1999 by Radiation Research Society.