M. Durante et al., Association between G(2)-phase block and repair of radiation-induced chromosome fragments in human lymphocytes, RADIAT RES, 151(6), 1999, pp. 670-676
We have studied the induction of chromosomal aberrations in human lymphocyt
es exposed in G(0) to X rays or carbon ions. Aberrations were analyzed in G
(0), G(1), G(2) or M phase. Analysis during the interphase was performed by
chemically induced premature chromosome condensation, which allows scoring
of aberrations in G(1), G(2) and M phase; fusion-induced premature chromos
ome condensation was used to analyze the damage in G(0) cells after incubat
ion for repair; M-phase cells were obtained by conventional Colcemid block.
Aberrations were scored by Giemsa staining dr fluorescence in situ hybridi
zation (chromosomes 2 and 4). Similar yields: of fragments were observed in
G(1) and G(2) phase, but lower yields were scored in metaphase,The frequen
cy of chromosomal exchanges was similar in G(0) (after repair), G(2) and M
phase for cells exposed to X rays, while a lower frequency of exchanges was
observed in M phase when lymphocytes were irradiated with high-LET carbon
ions,The results suggest that radiation-induced G(2)-phase block is associa
ted with unrejoined chromosome fragments induced by radiation exposure duri
ng G(0). (C) 1999 by Radiation Research Society.