The microhardness of enamel, primary dentine and secondary dentine was dete
rmined in the incisor teeth of 39 horses of three different breeds, trotter
horses, Belgian draft horses and Arab horses. Using a microhardness tester
fitted with a Knoop diamond indenter, the overall Knoop Hardness Number wa
s determined for each tissue, and the influences of breed and age on the ha
rdness were evaluated. Enamel and secondary dentine were significantly hard
er in Arab horses than in trotters and Belgian draft horses, but there were
no significant differences between draft horses and trotter horses in the
hardness of their enamel and dentine.