HIGH GLUCOSE AND HYPEROSMOLARITY INCREASE SECRETION OF INTERLEUKIN-1-BETA IN CULTURED HUMAN AORTIC ENDOTHELIAL-CELLS

Interleukin-1 (IL-1) is secreted by endothelial cells (ECs) and smooth -muscle cells (SMCs), which are two major component cells of vessels a nd detected in atherosclerotic lesions. To evaluate the effect of hype rglycemia on the secretion of IL-1 beta in endothelial cells in diabet ic patients, we investigated the effects of high glucose and hyperosmo lar conditions on the secretion of IL-1 beta from cultured human aorti c endothelial cells (HAECs). HAECs were treated with high concentratio n of glucose or hyperosmolar condition for 3 days. IL-1 beta in the su pernatant was measured by high sensitive enzyme-linked immunosorbent a ssay (ELISA). Under high concentration of glucose (16.6 mmol/L) and hy perosmolar condition (glucose 5.5 mmol/L + mannitol 11.1 mmol/L), the secretion of IL-1 beta was significantly increased (41.0 +/- 2.8 and 2 6.3 +/- 5.9% increase, respectively, compared with that of 5.5 mmol/L glucose). In conclusion, high glucose and hyperosmolar condition incre ase the secretion of IL-1 beta in HAECs. The results suggest that diab etic macroangiopathies might be accelerated partly through the increas e of IL-1 beta secretion in HAECs. (C) Elsevier Science Inc., 1997.