Malignant human gliomas express an amiloride-sensitive Na+ conductance

Citation
Jk. Bubien et al., Malignant human gliomas express an amiloride-sensitive Na+ conductance, AM J P-CELL, 45(6), 1999, pp. C1405-C1410
Citations number
34
Categorie Soggetti
Cell & Developmental Biology
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
ISSN journal
03636143 → ACNP
Volume
45
Issue
6
Year of publication
1999
Pages
C1405 - C1410
Database
ISI
SICI code
0363-6143(199906)45:6<C1405:MHGEAA>2.0.ZU;2-3
Abstract
Human astrocytoma cells were studied using whole cell patch-clamp recording . An inward, amiloride-sensitive Na+ current was identified in four continu ous cell lines originally derived from human glioblastoma cells (CH235, CRT , SKMG-1, and U251-MG) and in three primary cultures of cells obtained from glioblastoma multiforme tumors (up to 4 passages). In addition, cells fres hly isolated from a resected medulloblastoma tumor displayed this same char acteristic inward current. In contrast, amiloride-sensitive currents were n ot observed in normal human astrocytes, low-grade astrocytomas, or juvenile pilocytic astrocytomas. The only amiloride-sensitive Na+ channels thus far molecularly identified in brain are the brain Na+ channels (BNaCs). RT-PCR analyses demonstrated the presence of mRNA for either BNaC1 or BNaC2 in th ese tumors and in normal astrocytes. These results indicate that the functi onal expression of amiloride-sensitive Na+ currents is a characteristic fea ture of malignant brain tumor cells and that this pathway may be a potentia lly useful target for therapeutic intervention.