A potentiometric biosensor for D-amygdalin based on a consolidated biocomposite membrane

A new potentiometric biosensor for D-amygdalin based on a consolidated bioc omposite membrane has been designed. The manufacturing technique involved a simple compression of dry components presenting an ideal technology for th e mass production of potentiometric biosensor devices. The effect of high p ressures applied during the preparation of a D-amygdalin biosensor membrane was studied. The immobilisation of beta-glucosidase was improved by increa sing this pressure. The optimal biocomposite membrane composition was 100 m g electroactive salts (AgI, Ag2S) and 10 mg enzyme (50 U). Characteristics such as linear range, response times and degree of enzyme immobilisation we re studied for each of the pressures used in the production of the membrane s. (C) 1999 Elsevier Science B.V. All rights reserved.