Gr. Pettit et al., Antineoplastic agents. Part 409: Isolation and structure of montanastatin from a terrestrial actinomycete, BIO MED CH, 7(5), 1999, pp. 895-899
A Montana soil actinomycete, Streptomyces anulatus, produced (1x10(-2)% yie
ld) a new cancer cell growth inhibitory cyclooctadepsipeptide named montana
statin (1) accompanied by the potent anticancer antibiotic valinomycin (2)
in very high (5.1%) yields. Valinomycin but not montanastatin inhibited gro
wth of a number of pathogenic bacteria and fungi. Interpretation of high-fi
eld (500 MHz) NMR and high-resolution FAB mass spectral data allowed assign
ment of the structure cyclo-(D-Val-L-Lac-L-Val-D-Hiv) to montanastatin. Val
inomycin (2) was also isolated from actinomycetes cultured from a tree bran
ch and animal feces collected in Malaysia. Streptomyces exfoliatus, isolate
d from the tree branch, was found to contain valinomycin in 1.6% yield, whi
le the fecal isolate, S. anulatus, gave valinomycin in 0.9% yield. (C) 1999
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