Synthesis of polyamine derivatives for the preparation of affinity chromatography columns for the search of new Trypanosoma cruzi targets

The most potent trypanocidal compound of a series of symmetrically substitu ted 1,4-bis(3-aminopropylpiperazines) which displayed an IC50 value of 5 mu M On Trypanosoma cruzi trypomastigotes, was inactive on trypanothione redu ctase. Two derivatives 6 and 12 of this compound, one symmetrical and one d issymmetrical, were synthesized via a reductive amination reaction, to prep are affinity chromatography columns, which allowed us to isolate three para sitic proteins. Among these, the major ligand 6- and 12-binding protein hav ing an apparent molecular weight of 52 kDa has been identified as the thiol -disulfide oxido-reductase Tc52, previously characterized in Trypanosoma cr uzi. (C) 1999 Elsevier Science Ltd. All rights reserved.