Ch. Schilling et al., Metabolic pathway analysis: Basic concepts and scientific applications in the post-genomic era, BIOTECH PR, 15(3), 1999, pp. 296-303
This article reviews the relatively short history of metabolic pathway anal
ysis. Computer-aided algorithms for the synthesis of metabolic pathways are
discussed. Important algebraic concepts used in pathway analysis, such as
null space and convex cone, are explained. It is demonstrated how these con
cepts can be translated into meaningful metabolic concepts. For example, it
is shown that the simplest vectors spanning the region of all admissible f
luxes in stationary states, for which the term elementary flux modes was co
ined, correspond to fundamental pathways in the system. The concepts are il
lustrated with the help of a reaction scheme representing the glyoxylate cy
cle and adjacent reactions of aspartate and glutamate synthesis. The interr
elations between pathway analysis and metabolic control theory are outlined
. Promising applications for genome annotation and for biotechnological pur
poses are discussed. Armed with a better understanding of the architecture
of cellular metabolism and the enormous amount of genomic data available to
day, biochemists and biotechnologists will be able to draw the entire metab
olic map of a cell and redesign it by rational and directed metabolic engin
eering.