Effect of a cachectic factor on carbohydrate metabolism and attenuation byeicosapentaenoic acid

Citation
Hj. Hussey et Mj. Tisdale, Effect of a cachectic factor on carbohydrate metabolism and attenuation byeicosapentaenoic acid, BR J CANC, 80(8), 1999, pp. 1231-1235
Citations number
19
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
BRITISH JOURNAL OF CANCER
ISSN journal
00070920 → ACNP
Volume
80
Issue
8
Year of publication
1999
Pages
1231 - 1235
Database
ISI
SICI code
0007-0920(199906)80:8<1231:EOACFO>2.0.ZU;2-3
Abstract
The effect of a proteolysis-inducing factor (PIF), produced by cachexia-ind ucing tumours on glucose utilization by different tissues and the effect of pretreatment with the polyunsaturated fatty acid eicosapentaenoic acid (EP A), has been determined using the 2-deoxyglucose tracer technique. Mice rec eiving PIF showed a profound depression of body weight (2.3 g) over a 24-h period, which was completely abolished by pretreatment with a monoclonal an tibody to PIF or by 3 days pretreatment with EPA at 500 mg kg(-1). Animals receiving PIF exhibited a marked hypoglycaemia, which was effectively rever sed by both antibody and EPA pretreatment. There was an increase in glucose utilization by brain, heart and brown fat, but a decrease by kidney, white fat, diaphragm and gastrocnemius muscle after administration of PIF. Chang es in organ glucose consumption were attenuated by either monoclonal antibo dy, EPA, or both. There was a decrease in 2-deoxyglucose uptake by C2C12 my oblasts in vitro, which was attenuated by EPA. This suggests a direct effec t of PIF on glucose uptake by skeletal muscle. These results suggest that i n addition to a direct catabolic effect on skeletal muscle PIF has a profou nd effect on glucose utilization during cachexia.