Application of thylakoids in herbicide-detecting system

A simple method of the photosynthesis inhibiting herbicides detecting is pr oposed as practical approach for environmental monitoring. Method combines the microtiter-plate assay based on the inhibition of the thylakoid membran e activity with the sample preconcentrating on the solid-phase cartridges. The C-18 cartridge provides acceptable recovery (>85%) of atrazine, simazin e and cyanazine in broad range of concentrations. The method proposed allow s detecting the photosynthesis inhibiting herbicides in the concentration r ange of 0.025-25 mu g/l. To improve system stability thylakoid immobilizati on in poly(vinylalcohol) bearing styrylpyridinium groups.(PVA-SbQ) on micro titer plates is performed.