A simple method of the photosynthesis inhibiting herbicides detecting is pr
oposed as practical approach for environmental monitoring. Method combines
the microtiter-plate assay based on the inhibition of the thylakoid membran
e activity with the sample preconcentrating on the solid-phase cartridges.
The C-18 cartridge provides acceptable recovery (>85%) of atrazine, simazin
e and cyanazine in broad range of concentrations. The method proposed allow
s detecting the photosynthesis inhibiting herbicides in the concentration r
ange of 0.025-25 mu g/l. To improve system stability thylakoid immobilizati
on in poly(vinylalcohol) bearing styrylpyridinium groups.(PVA-SbQ) on micro
titer plates is performed.