Expression of the transmembrane protein tyrosine phosphatase RPTP alpha inhuman oral squamous cell carcinoma

Little is known about the role of protein-tyrosine phosphatases (PTPs), the cellular counterparts of protein-tyrosine kinases, both for normal growth regulation and for its dysregulation in cancer. The receptor-like PTP alpha (RPTP alpha) may play a positive role in growth regulation and has been sh own to be overexpressed in colon carcinoma. An RNA/RNA in situ hybridisatio n protocol for RPTP alpha as well as RPTP alpha immunohistochemistry was de veloped to evaluate RPTP alpha expression in oral squamous cell carcinomas (OSCCs) of different histological grade and to reveal the synthetically act ive cells and their tissue distribution. In well-differentiated OSCC (G1), RPTP alpha mRNA could be detected by in situ hybridisation exclusively in s troma cells (fibro/myofibroblasts and inflammatory cells). A higher histolo gical grade (G2/G3) was associated with an increased number of RPTP alpha-s ynthesising carcinoma cells haphazardly distributed within invading tumour areas. Consistent results were obtained by immunocytochemistry. Thus, both carcinoma dedifferentiation and stroma recruitment and activation seem to b e associated with an upregulation of RPTP alpha expression in OSCC. The res ults speak in favour of the important role of activation of stroma fibro/my ofibroblasts influencing the biological behaviour of epithelial tumours and also suggest that elevated RPTP alpha expression may be a more general mar ker for proliferating or dedifferentiated cells.