Vv. Demidov et al., KINETIC-ANALYSIS OF SPECIFICITY OF DUPLEX DNA TARGETING BY HOMOPYRIMIDINE PEPTIDE NUCLEIC-ACIDS, Biophysical journal, 72(6), 1997, pp. 2763-2769
A simple theoretical analysis shows that specificity of double-strande
d DNA (dsDNA) targeting by homopyrimidine peptide nucleic acids (hpyPN
As) is a kinetically controlled phenomenon. Our computations give the
optimum conditions for sequence-specific targeting of dsDNA by hpyPNAs
. The analysis shows that, in agreement with the available experimenta
l data, kinetic factors play a crucial role in the selective targeting
of dsDNA by hpyPNAs. The selectivity may be completely lost if PNA co
ncentration is too high and/or during prolonged incubation of dsDNA wi
th PNA. However, quantitative estimations show that the experimentally
observed differences in the kinetic constants for hpyPNA binding with
the correct and mismatched DNA sites are sufficient for sequence-spec
ific targeting of long genomic DNA by hpyPNAs with a high yield under
appropriate experimental conditions. Differential dissociation of hpyP
NA/dsDNA complexes is shown to enhance the selectivity of DNA targetin
g by PNA.