Heart transplant rejection is routinely defined by histological evaluation
of endomyocardial biopsies (EMB). As elevated levels of donor derived sHLA
dsHLA can be detected in the serum of transplanted patients just before or
during rejection, quantification of donor specific soluble counterparts of
HLA. Class I(sHLA-I) in the serum of the recipient may be a new way for non
-invasive monitoring of graft rejection. However, not all patients show an
increase of dsHLA at time of rejection. A reason for this might be that ant
i-donor-HLA antibodies, which are formed by the patient, form complexes wit
h donor sHLA-I molecules. This masking or blocking of sHLA-I binding sites
might cause false-negative results of tests detecting donor specific sHLA.
Using HLA-antigen specific ELISA tests we could demonstrate that most anti-
HLA antibodies block the detection of sHLA antigens in plasma, even in high
dilutions of the antibody when the antibodies were not detectable in a CDC
test. In general, HLA-antigen specific antibodies block the detection of s
HLA molecules, while broadly-reactive antibodies, recognizing another epito
pe on the molecule, do not. The implication of these findings is that more
than one dsHLA allotype within one patient should be tested to monitor graf
t rejection. In addition, sHLA monitoring must be combined with an HLA-anti
body screening. (C) American Society for Histocompatibilicy and Immunogenet
ics, 1999 Published by Elsevier Science Inc.