Specific sHLA in healthy donors and donor-specific sHLA in renal transplant patients

We studied cadaver kidney transplant recipients to determine if their serum levels of donor-specific class I sHLA correlated with graft outcome. Testi ng of sHLA was performed by an ELISA sandwich assay using allospecific mono clonal trapping antibodies and anti-beta 2-mu detecting antibody. Sufficien t sHLA sensitivity (<1 ng:ml) was achieved by using two synergistic trappin g antibodies. Suitable antibodies were available for A2 and B7, and data we re collected for these two antigens. Stability of these sHLA was determined in plasma and serum as were ranges of normal and background levels. Backgr ound levels varied substantially. Five AZ recipients of A2(+) grafts and 5 B7(-) recipients of B7(+) grafts were studied with appropriate sHLA levels measured pretransplant and at intervals post-transplant. Graft outcome was assessed by serum creatinines? renal biopsies and/or therapy for rejection. in the 5 patients (3 A2(-) and 2 B7(-)) whose post-transplant donor-specif ic sHLA never exceeded immunological complications (e.g., post-operative AT N, ureteral obstruction) did not affect the correlation. In the 5 patients with post-transplant levels exceeding pre-transplant levels, subsequent evi dence of rejection was observed. Periodic measurement of donor-specific sHL A should be a useful instrument for monitoring renal allograft rejection. ( C) American Society for Histocompatibility and Immunogenetics, 1999 Publish ed by Elsevier Science Inc.