PEPTIC PEPTIDE-MAPPING BY HPLC, ON LINE WITH PHOTODIODE-ARRAY DETECTION, OF A HEMOGLOBIN HYDROLYSATE PRODUCED AT PILOT-PLANT SCALE FROM AN ULTRAFILTRATION PROCESS

The analysis of a peptic bovine hemoglobin hydrolysate, produced at pi lot plant scale, was carried out using two techniques: SE-HPLC and RP- HPLC. Analysis of amino acid composition, second-order derivative spec trometry and FAB mass spectrometry of isolated peptides allowed us to determine the exact positions of these peptides in the sequence of bov ine hemoglobin. This, consequently, gave rise to a peptidic map of the hydrolysate. It also revealed, at the same time, some biologically ac tive peptides in the hydrolysate. This information should find use in the potential future application of enzymatic bovine hemoglobin hydrol ysate.