Evaluation of the influence of N-nitrosodimethyloamine (NDMA) on the apoptosis of neutrophils of peripheral blood (PMN) and the expression of the IL-6R membrane receptor-in vitro research

Evaluation of the influence of N-nitrosodimethyloamine (NDMA) on the apopto sis of neutrophils of peripheral blood (PMN) and the expression of the IL-6 R membrane receptor - in vitro research. The aim of the present work was the evaluation of N-nitrosodimethyloamine ( NDMA) on the induction of apoptosis in the neutrophils of peripheral blood as well as the evaluation of the surface receptors for IL-6. The isolated n eutrophils were incubated for 1 and 3 hours with NDMA of a concentration of 2.5, 5, 7.5, and 10 mg/ml. In the samples incubated for 1 hour a significa nt, dose- dependent increase of apoptosis in the examined cells was observe d. In the cells incubated for 3 hours, the increase of apoptosis was observ ed only at concentration of NDMA of 2.5 and 5 mg/ml. In case of higher conc entration used, probably necrotic processes dominated in the cells. No infl uence of NDMA on the expression of IL-6R was observed.