OPTIMIZATION OF A REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SEPARATION USING AN ION-PAIR REAGENT FOR THE DETERMINATION OF CARBOXYLIC-ACIDS IN PLANT MATERIALS
Cf. Harrington et al., OPTIMIZATION OF A REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SEPARATION USING AN ION-PAIR REAGENT FOR THE DETERMINATION OF CARBOXYLIC-ACIDS IN PLANT MATERIALS, Journal of liquid chromatography & related technologies, 20(11), 1997, pp. 1773-1787
Citations number
23
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A method was developed to separate a number of mono-, di- and tri- car
boxylic acids present in plant materials, using a C-18 column and a si
mple eluent containing a phosphate buffer and tetra-n-butylammonium ph
osphate, an ion-pairing agent. The effects of the ion-pairing agent, p
H, presence or absence of organic modifier and the concentration of th
e buffer, on the chromatographic response function (CRF), were investi
gated using a two level factorial experimental design. The results ind
icated that a number of main, two and three factor effects were import
ant. The largest effect was due to the interaction of pH with ion-pair
ing concentration and was further investigated using univariate experi
ments. The mobile phase developed consisted of 10 mM tetra-n-butylammo
nium phosphate and 20 mM potassium dihydrogen orthophosphate at pH 3.3
. Formic, glutamic, quinic, succinic, malic and tartaric acids were ex
tracted from 3 different grass ecotypes, the extracts were cleaned-up
using a C-18 Sep-Pak(R) cartridge and then identified using the develo
ped procedure.