Ascorbic acid in buffalo ovary in relation to oestrous cycle

Concentration of ascorbic acid was determined in different parts of buffalo ovary at four different stages of oestrous cycle viz. early luteal, mid lu teal, late luteal and follicular, The stages were decided from the physical and morphological examinations of corpora lutea. The ovary was dissected i n three components viz. corpus luteum, follicular fluid and ovarian stromal tissue for ascorbic acid assay. Corpus luteum showed significant change in concentration of ascorbic acid with the advancement of oestrous cycle, val ue being highest in late luteal stage. Follicular fluid and ovarian stromal tissue did not show significant changes in ascorbic acid at any stage of t he oestrous cycle. Small follicles, irrespective of the stage of oestrous c ycle had, however, significantly higher ascorbic acid content than large fo llicles.