Concentration of ascorbic acid was determined in different parts of buffalo
ovary at four different stages of oestrous cycle viz. early luteal, mid lu
teal, late luteal and follicular, The stages were decided from the physical
and morphological examinations of corpora lutea. The ovary was dissected i
n three components viz. corpus luteum, follicular fluid and ovarian stromal
tissue for ascorbic acid assay. Corpus luteum showed significant change in
concentration of ascorbic acid with the advancement of oestrous cycle, val
ue being highest in late luteal stage. Follicular fluid and ovarian stromal
tissue did not show significant changes in ascorbic acid at any stage of t
he oestrous cycle. Small follicles, irrespective of the stage of oestrous c
ycle had, however, significantly higher ascorbic acid content than large fo
llicles.