Alterations in pulmonary surfactant after rapid arousal from torpor in themarsupial Sminthopsis crassicaudata

Citation
Ov. Lopatko et al., Alterations in pulmonary surfactant after rapid arousal from torpor in themarsupial Sminthopsis crassicaudata, J APP PHYSL, 86(6), 1999, pp. 1959-1970
Citations number
33
Categorie Soggetti
Physiology
Journal title
JOURNAL OF APPLIED PHYSIOLOGY
ISSN journal
87507587 → ACNP
Volume
86
Issue
6
Year of publication
1999
Pages
1959 - 1970
Database
ISI
SICI code
8750-7587(199906)86:6<1959:AIPSAR>2.0.ZU;2-C
Abstract
Torpor in the dunnart, Sminthopsis crassicaudata, alters surfactant lipid c omposition and surface activity. Here we investigated changes in surfactant composition and surface activity over 1 h after rapid arousal from torpor (15-30 degrees C at 1 degrees C/min). We measured total phospholipid (PL), disaturated PL (DSP), and cholesterol (Chol) content of surfactant lavage a nd surface activity (measured at both 15 and 37 degrees C in the captive bu bble surfactometer). Immediately after arousal, Chol decreased (from 4.1 +/ - 0.05 to 2.8 +/- 0.3 mg/g dry lung) and reached warm-active levels by 60 m in after arousal. The Chol/DSP and Chol/PL ratios both decreased to warm-ac tive levels 5 min after arousal because FL, DSP, and the DSP/PL ratio remai ned elevated over the 60 min after arousal. Minimal surface tension and fil m compressibility at 17 mN/m at 37 degrees C both decreased 5 min after aro usal, correlating with rapid changes in surfactant Chol. Therefore, changes in lipids matched changes in surface activity during the postarousal perio d.