Discrete analysis of serum uric acid with immobilized uricase and peroxidase

Commercially available uricase and peroxidase have been immobilized onto al kylamine glass and arylamine glass beads respectively. A discrete method ha s been developed to determine uric acid in serum using immobilized uricase and peroxidase. The method is based on generation of H2O2 from serum uric a cid by immobilized uricase and its measurement by a colour reaction catalyz ed by immobilized peroxidase. The minimum detection limit of the method was 8 mu g/0.1 mi sample. The mean analytical recovery of added uric acid in s erum was 87.5%. The within and between assay coefficient of variation (C.V. ) were <6.58% and <10.77% respectively. The serum uric acid in apparently h ealthy adults and persons suffering from different disease was found to be 25-55 mu g/ml, 32+/-2.25 (range, mean+/-S.D.) and 55-200 mu g/ml; 52+/-6.4 (range, mean+/-S.D.) respectively by our method. A good correlation (r = 0. 8170) was obtained between the serum urate values by this method and with t hose obtained by commercial Enzo-kit method. (C) 1999 Elsevier Science B.V. All rights reserved.