Quantitative determination of free intracellular amino acids in single human polymorphonuclear leucocytes - Recent developments in sample preparationand high-performance liquid chromatography
J. Muhling et al., Quantitative determination of free intracellular amino acids in single human polymorphonuclear leucocytes - Recent developments in sample preparationand high-performance liquid chromatography, J CHROMAT B, 728(2), 1999, pp. 157-166
The described procedure allows quantitative, highly precise and reproducibl
e analysis of free amino acid concentrations in single polymorphonuclear le
ucocytes (PMLs). This method is superior to previously described procedures
with regard to sample size, PML separation, sample preparation and stabili
ty, as well as the chosen fluorescence high-performance liquid chromatograp
hy procedure, and can satisfy the high demands for ultra-sensitive and comp
rehensive amino acid analysis, especially for the continuous surveillance o
f severe diseases and organ dysfunction. (C) 1999 Elsevier Science B.V. All
rights reserved.