Quantitative determination of free intracellular amino acids in single human polymorphonuclear leucocytes - Recent developments in sample preparationand high-performance liquid chromatography

The described procedure allows quantitative, highly precise and reproducibl e analysis of free amino acid concentrations in single polymorphonuclear le ucocytes (PMLs). This method is superior to previously described procedures with regard to sample size, PML separation, sample preparation and stabili ty, as well as the chosen fluorescence high-performance liquid chromatograp hy procedure, and can satisfy the high demands for ultra-sensitive and comp rehensive amino acid analysis, especially for the continuous surveillance o f severe diseases and organ dysfunction. (C) 1999 Elsevier Science B.V. All rights reserved.