High-performance liquid chromatography determination of residue levels on chicken carcasses treated with cetylpyridinium chloride

Cetylpyridinium chloride (CPC) has been found to be effective in reducing c ontamination of chicken carcasses from a variety of microorganisms, includi ng Escherichia coli O157:H7, Salmonella typhimurium, Campylobacter jejuni, Aeromonas hydrophila, Listeria monocytogenes, and Staphylococcus aureus. A procedure has been developed to determine residue levels on chicken carcass es after CPC treatment. For the analysis, chicken carcasses were extracted with 95% ethanol. The CPC concentration in the extract was measured by high -performance liquid chromatography (HPLC) with ultraviolet detection using dodecylpyridinium chloride (:DPC) as an internal standard. The method was v alidated in the concentration range of 3-200 mu g/ml CPC in ethanolic extra ct. This assay is rapid, precise, and accurate. (C) 1999 Published by Elsev ier Science B.V. All rights reserved.