An oligonucleotide-ligation assay for the differentiation between Cyclospora and Eimeria spp. polymerase chain reaction amplification products

Authors
Jinneman, KC Wetherington, JH Hill, WE Omiescinski, CJ Adams, AM Johnson, JM Tenge, BJ Dang, NL Wekell, MM
Citation
Kc. Jinneman et al., An oligonucleotide-ligation assay for the differentiation between Cyclospora and Eimeria spp. polymerase chain reaction amplification products, J FOOD PROT, 62(6), 1999, pp. 682-685
Citations number
12
Categorie Soggetti
Food Science/Nutrition
Journal title
JOURNAL OF FOOD PROTECTION
ISSN journal
0362028X → ACNP
Volume
62
Issue
6
Year of publication
1999
Pages
682 - 685
Database
ISI
SICI code
0362-028X(199906)62:6<682:AOAFTD>2.0.ZU;2-A
Abstract
An oligonucleotide-ligation assay (OLA) was developed and compared to a res triction fragment length polymorphism (RFLP) test for distinguishing betwee n 294-bp polymerase chain reaction (PCR) amplification products of the 18S rRNA gene from Cyclospora and Eimeria spp. The PCR/OLA correctly distinguis hed between three Cyclospora, three E. tenella, and one E. mitis strains an d the ratio of positive to negative spectrophotometric absorbance (A(490)) values for each strain ranged from 4.086 to 15.280 (median 9.5). PCR/OLA pr ovides a rapid, reliable, spectrophotometric alternative to PCR/RFLP.