Cytokine production by CD4+T-cells responding to antigen presentation by melanoma cells

Citation
Ms. Brady et al., Cytokine production by CD4+T-cells responding to antigen presentation by melanoma cells, MELANOMA RE, 9(2), 1999, pp. 173-180
Citations number
37
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
MELANOMA RESEARCH
ISSN journal
09608931 → ACNP
Volume
9
Issue
2
Year of publication
1999
Pages
173 - 180
Database
ISI
SICI code
0960-8931(199904)9:2<173:CPBCRT>2.0.ZU;2-X
Abstract
Melanoma cells are unusual because, unlike most epithelial tumours, constit utive expression of HLA class II antigens is common. We have previously dem onstrated that a peptide-specific CD4+ 7-cell clone proliferates briskly in response to peptide and HLA class II expressing melanoma cell lines derive d from metastases. Here we demonstrate that these CD4+ T-cells secrete larg e amounts of interferon-gamma (IFN gamma) and interleukin-10 (IL10), and in significant quantities of IL2 or IL4, in response to peptide presentation b y both melanoma and autologous B-cells. T-cells produced more IL10 when res ponding to peptide presentation by melanoma cells compared with B-cells, an d less IFN gamma (P<0.01). Addition of IL12 did not alter the cytokines pro duced but increased the T-cell production of both, especially the productio n of IL10 in response to peptide presentation by melanoma cells. Our data s uggest that differential cytokine production by CD4+ T-cells in response to peptide presentation by HLA class II expressing tumour cells may contribut e to tolerance to tumour antigens. (C) 1999 Lippincott Williams & Wilkins.