Quantitative phytochemical analyses of six Hypericum species growing in Slovenia

The 74 samples of six Hypericum species (H. perforatum, H. hirsutum, H. mac ulatum, H. tetrapterum, H. montanum, and H. humifusum) were collected aroun d Slovenia and analysed for the content of ten substances (rutin, hyperosid e, isoquercetin, quercitrin, quercetin, 13,118-biapigenin, amentoflavone, p seudohypericin, hypericin, and hyperforin). The flowers were analysed separ ately from the green parts of the plants (herbs). The highest content of mo st of the substances was found in the flowers of H, perforatum. Among the h erbal samples (without flowers), H. montanum and H. hirsutum contained sign ificantly higher levels of amentoflavone (average 3-fold and 2.5-fold highe r, respectively), than the herbs of H. perforatum. In the herbal part of H. perforatum the contents of all constituents strongly correlate with the co ntents of the same compound in flowers, except for the content of amentofla vone, which is independent in these two parts. Rutin and hyperoside are in positive correlation, and quercitrin is in negative correlation with the al titude of the growing site.