Synthesis and enzymatic incorporation of morpholino thymidine-5 '-triphosphate in DNA fragments

Authors
Marciacq, F Sauvaigo, S Issartel, JP Mouret, JF Molko, D
Citation
F. Marciacq et al., Synthesis and enzymatic incorporation of morpholino thymidine-5 '-triphosphate in DNA fragments, TETRAHEDR L, 40(25), 1999, pp. 4673-4676
Citations number
15
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON LETTERS
ISSN journal
00404039 → ACNP
Volume
40
Issue
25
Year of publication
1999
Pages
4673 - 4676
Database
ISI
SICI code
0040-4039(19990618)40:25<4673:SAEIOM>2.0.ZU;2-5
Abstract
4-(carboxymethyl)-2-(thymidin-9-yl)-6-(hydroxymethyl)morpholine-6-triphosph ate (morpholino thymidine-5'-triphosphate) was synthesised from 1-(beta-D-e rythro-pentofuranosyl) thymine. It was fully characterised by NMR, UV and m ass spectrometry. Taq polymerase enzymatic incorporation of this nucleotide analogue into DNA fragments was investigated. Morpholino thymidine-5'-trip hosphate was incorporated in a base-specific process and acted as a novel c hain terminator in DNA sequencing, similarly to the corresponding dideoxynu cleotide. (C) 1999 Elsevier Science Ltd. All rights reserved.