Characterization of a newly found stretch-activated K-Ca,K-ATP channel in cultured chick ventricular myocytes

With the use of the patch-clamp technique, five kinds of stretch-activated (SA) ion channels were identified on the basis of their single-channel cond uctances and ion selectivities in cultured chick ventricular myocytes. Beca use a high-conductance K+-selective channel predominated among these channe ls, we concentrated on characterizing its properties mostly using excised i nside-out patches. With 145 mM KCl solution in the pipette and the bath, th e channel had a conductance of 199.8 +/- 8.2 pS (n = 22). The ion selectivi ties among K+, Na+, Ca2+, and Cl- as estimated from their permeability rati os were P-Na/P-K = 0.03, P-Ca/P-K = 0.025, and P-Cl/P-K = 0.026. The probab ility of the channel being open (P-0) increased with the Ca2+ concentration in the bath ([Ca2+](b); dissociation constant Kd = 0.51 mu M at +30 mV) an d membrane potential (voltage at half-maximal P-o, = 39.4 mV at 0.35 FIM [C a2+](b)). The channel was blocked by gadolinium, tetraethylammonium, and ch arybdotoxin from the extracellular surface and, consequently, was identifie d as a Ca2+-activated K+ (K-Ca) channel type. The channel was also reversib ly activated by ATP applied to the intracellular surface (K-d = 0.74 mM at 0.10 mu M [Ca2+](b) at + 30 mV). From these data taken together, we conclud ed that the channel is a new type of K-Ca channel that could be designated as an "SA K-Ca,K-ATP channel. "To our knowledge, this is the first report o f K-Ca channel in heart cells.