Tf. Allred et al., Brief 95% O-2 exposure effects on surfactant protein and mRNA in rat alveolar and bronchiolar epithelium, AM J P-LUNG, 20(6), 1999, pp. L999-L1009
Citations number
35
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
In acute lung injury, a disturbed surfactant system may impair gas exchange
. Previous evaluations of hyperoxia effects on surfactant proteins (SPs) fo
llowed exposures >1-2 days. To evaluate the effects of brief exposure to hy
peroxia on the SP system, we exposed adult male rats to 95% O-2 or air for
12, 36, and 60 h. SP-A, -B, and -C mRNAs were analyzed by Northern blot and
semiquantitative in situ hybridization (ISH). SP-A and -B were analyzed in
whole lung homogenates, lung lavage fluid, and fixed tissue by semiquantit
ative immunohistochemistry (IHC). All SP mRNAs were diminished at 12 h and
rose to or exceeded control by 60 h as determined by Northern blot and ISH.
These effects were seen mainly in the intensity of ISH signal per cell in
both type II and bronchiolar epithelial (Clara) cells and to a lesser exten
t on numbers of positively labeled cells. SP-B declined to 50% of control i
n lavage at 12 h, but no changes in total lung SP-A and -B were seen. The n
umber of SP-A positively labeled cells did not change, but SP-A label inten
sity measured by IHC in type II cells showed parallel results to Northern b
lots and ISH. The response of SP-A in Clara cells was similar. SP-B immunol
abeling intensity rose in both type II and Clara cells throughout the expos
ure. SP-C ISH intensity fell at 12 h and was increased to two times control
by 60 h of hyperoxia. Sharp declines in SP expression occurred by 12 h of
95% O-2 and may affect local alveolar stability.