Characterization of Lactobacillus collinoides response to heat, acid and ethanol treatments

Tolerance to stress and cross-protection in Lactobacillus collinoides were examined after exposure to ethanol, acid or heat shock. Ethanol and heat-ad apted cells demonstrate induced homologous tolerance and cross-resistance t o acid stress. No cross-protection of acid-adapted cells against ethanol an d heat stresses was observed. Heat was the only pretreatment leading to cro ss-protection against the two other stresses. Whole-cell protein extract an alysis revealed that each treatment induced a battery of stress proteins; t he synthesis of some of these polypeptides being induced by more than one c ondition. The greatest overlap was observed between ethanol and heat treatm ents. Ten proteins were found to be common to these stresses.