Interleukin 1 beta, interleukin 6, beta(2)-microglobulin, and transforminggrowth factor-alpha in gingival crevicular fluid from human periodontal disease
M. Mogi et al., Interleukin 1 beta, interleukin 6, beta(2)-microglobulin, and transforminggrowth factor-alpha in gingival crevicular fluid from human periodontal disease, ARCH ORAL B, 44(6), 1999, pp. 535-539
Inflammatory mediators are central to the pathogenesis of periodontal disea
ses and may be used as markers in diagnosis. The aim of this study was to i
dentify and quantify the various growth factors, apoptosis-related modifier
s [soluble form of Fas (sFas) and bcl-2] and cytokines in the gingival crev
icular fluid (GCF) of patients with different severities of periodontitis a
s compared with those of controls. GCF samples were taken from patients wit
h periodontal disease and from controls. The concentrations of epidermal gr
owth factor, transforming growth factor (TGF)-alpha, interleukin (IL)-1 bet
a, IL-6, interferon-gamma, beta(2)-microglobulin (beta(2)-MG), and apoptosi
s-related modifiers sFas and bcl-2 in the samples were determined by enzyme
-linked immunosorbent assay. TGF-alpha was significantly lower in patients
with periodontal disease than in the controls. In contrast, the concentrati
ons of IL-1 beta, IL-6, and beta(2)-MG were significantly higher in the gro
up with severe periodontal disease than in the controls. The amount of tota
l protein in the GCF was considerably higher in the disease group than the
controls (p < 0.05). TGF-alpha, IL-1 beta, and beta(2)-MG concentrations we
re associated (Spearman rank correlation, r < 0.05 for all) with clinical m
easures of disease severity (pocket depth) and inflammation (bleeding when
probed). Apoptosis-related modifiers (sFas and bcl-2) could not be detected
in any samples. These results suggest that the growth factor TGF-alpha and
certain cytokines are associated with the presence of periodontal disease.
(C) 1999 Published by Elsevier Science Ltd. All rights reserved.