Interleukin 1 beta, interleukin 6, beta(2)-microglobulin, and transforminggrowth factor-alpha in gingival crevicular fluid from human periodontal disease

Citation
M. Mogi et al., Interleukin 1 beta, interleukin 6, beta(2)-microglobulin, and transforminggrowth factor-alpha in gingival crevicular fluid from human periodontal disease, ARCH ORAL B, 44(6), 1999, pp. 535-539
Citations number
25
Categorie Soggetti
da verificare
Journal title
ARCHIVES OF ORAL BIOLOGY
ISSN journal
00039969 → ACNP
Volume
44
Issue
6
Year of publication
1999
Pages
535 - 539
Database
ISI
SICI code
0003-9969(199906)44:6<535:I1BI6B>2.0.ZU;2-4
Abstract
Inflammatory mediators are central to the pathogenesis of periodontal disea ses and may be used as markers in diagnosis. The aim of this study was to i dentify and quantify the various growth factors, apoptosis-related modifier s [soluble form of Fas (sFas) and bcl-2] and cytokines in the gingival crev icular fluid (GCF) of patients with different severities of periodontitis a s compared with those of controls. GCF samples were taken from patients wit h periodontal disease and from controls. The concentrations of epidermal gr owth factor, transforming growth factor (TGF)-alpha, interleukin (IL)-1 bet a, IL-6, interferon-gamma, beta(2)-microglobulin (beta(2)-MG), and apoptosi s-related modifiers sFas and bcl-2 in the samples were determined by enzyme -linked immunosorbent assay. TGF-alpha was significantly lower in patients with periodontal disease than in the controls. In contrast, the concentrati ons of IL-1 beta, IL-6, and beta(2)-MG were significantly higher in the gro up with severe periodontal disease than in the controls. The amount of tota l protein in the GCF was considerably higher in the disease group than the controls (p < 0.05). TGF-alpha, IL-1 beta, and beta(2)-MG concentrations we re associated (Spearman rank correlation, r < 0.05 for all) with clinical m easures of disease severity (pocket depth) and inflammation (bleeding when probed). Apoptosis-related modifiers (sFas and bcl-2) could not be detected in any samples. These results suggest that the growth factor TGF-alpha and certain cytokines are associated with the presence of periodontal disease. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.