P. Vandoolaeghe et al., M-type 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is the productof a late muscle differentiation gene, BIOC BIOP R, 259(2), 1999, pp. 250-254
Citations number
23
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Genes that are expressed in adult muscle, but not in myotubes, are useful m
arkers of the last steps of muscle maturation. We have investigated at what
stage of differentiation the muscle-specific (M) promoter of a gene that c
odes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK2) become
s functional. M-PFK2 mRNA, which is present in adult muscle, did not appear
during differentiation of L6 myoblasts into myotubes induced by growth fac
tor withdrawal and hormonal treatment, even when this differentiation was s
timulated by expression of transgenes coding for myf-5 or Rb. A comparison
with the expression pattern of muscle genes showed that M-PFK2 is a marker
of mature skeletal muscle. We also found that M-PFK2 is expressed in both t
ypes (slow-twitch and fast-twitch) of adult muscle. Thus, the M-PFK2 promot
er is a novel model for studying the transcriptional control of the final s
teps of muscle differentiation that are common to the two types of myofiber
s. (C) 1999 Academic Press.