A structured, dynamic model for animal cell culture systems

There are a substantial number of applications for mathematical models in t he field of cell culture technology. A number of currently available animal cell culture models are identified and detailed. A structured model that i s capable of addressing a range of key applications is developed. This mode l considers some 50 components in three distinct regions (the medium, the c ytoplasm and the mitochondria) and includes descriptions of features such a s glycolysis, glutaminolysis, the TCA cycle, the formation and utilisation of fatty and amino acids, competitive trans-membrane transport, product for mation and cell growth and death. The overall model is modular in form and its structure is therefore amenable to expansion or adaptation to a range o f individual systems. (C) 1999 Elsevier Science S.A, All rights reserved.