A structured, dynamic model for animal cell culture systems: application to murine hybridoma

A detailed, structured animal cell model has a number of interesting potent ial applications. In this paper, a structured model will be 'tuned' to data for two different murine hybridoma cell lines, 2HG11 and AFP27. With an ap propriate set of parameters, the model was able to describe the behaviour o f both these cultures accurately. In order to demonstrate that the model co uld predict the behaviour of AFP27 cells under different conditions, a furt her AFP37 experiment was performed and its results compared to model predic tions, The model was capable of matching the behaviour of this culture usin g the parameters derived from the earlier data set. Differences between the predictions and the experimental data suggest regions in which either the model needs to be strengthened or in which the experimental data is questio nable. (C) 1999 Elsevier Science S.A. All rights reserved.